Microsatellite markers for the ectomycorrhizal basidiomycete Rhizopogon vinicolor
Annette M. Kretzer1,
Randy Molina2 and
Joseph W. Spatafora1
1Oregon State University, Department of Botany and Plant Pathology, 2082 Cordley Hall, Corvallis OR 973312902, USA
2USDA Forest Service Pacific North-west Research Station, 2300 SW Jefferson Way, Corvallis OR 97331, USA
Rhizopogon vinicolor is a false-truffle in the Boletales (Basidiomycota) and is a common ectomycorrhizal (EM) symbiont of Douglas-fir (Pseudotsuga menziesii) in the Pacific north-west of America (Molina et al. 1999). The ectomycorrhizae are tuberculate which means that they grow in dense clusters that are surrounded by a weft of darkly pigmented hyphae (Zak 1971). While much research has been conducted on population structure of fungal pathogens (McDonald 1997), knowledge on population structure of EM fungi is sparse, and most work has focused primarily on the size and distribution of genets (e.g. Dahlberg & Stenlid 1995). In this paper we describe the development of microsatellite markers for the EM basidiomycete R. vinicolor. Although R. vinicolor forms fruit-bodies below ground that are difficult to sample, R. vinicolor provides a number of advantages for population studies: (i) the tuberculate mycorrhizae are morphologically very distinct and provide ample material for DNA extraction and amplification; (ii) Rhizopogon species grow readily in culture; and (iii) they form mycorrhizae from either mycelia or spores under greenhouse conditions (Molina et al. 1999).
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