Isozyme patterns in Endocronartium harknessii from several pine hosts and stands in California.

Vogler, D. R., Kinloch, B. B., Jr., Cobb, F. W., Jr., and Popenuck, T. L. 1987.

Department of Plant Pathology, University of California, Berkeley, CA 94720, and
U.S.D.A., Forest Service, Institute of Forest Genetics, Berkeley, CA 94701.

Phytopathology 77:1242. (1987)


Abstract

Spores from single galls of Endocronartium harknessii (J. P. Moore) Hiratsuka [=Peridermium harknessii J. P. Moore] from native and non-native stands of Pinus radiata, P. muricata and P. coulteri along 640 km of coastal California were subjected to starch gel electrophoresis to ascertain isozyme banding patterns. Fifteen mg of sieved and air- dried spores of each field isolate were ground in 200 microliters of 0.1M phosphate buffer (pH 7.5) amended with 2% PVP, 2% PEG, 1% Tween 80, and 0.04% BSA, and the soluble extract separated electrophoretically on four gel-buffer systems (citrate-lithium borate, citrate-sodium borate, citrate, and morpholine citrate). Starch gels were stained for 27 enzymes, of which 19 resolved adequately for interpretation. Regardless of host or stand origin, most fungal enzymes tested were monomorphic.


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