Nuclei in mature aeciospores from two zymodemes of Peridermium harknessii in California.

Vogler, D. R., Cobb, F. W., Jr., and Epstein, L. 1989.

Department of Plant Pathology, University of California, Berkeley 94720.

Phytopathology 79:1164. (1989)


Abstract

We used epifluorescent microscopy to count nuclei in aeciospores from two zymodemes (electrophoretic types) of Peridermium harknessii J. P. Moore. For each single-gall isolate, spores were fixed overnight at 4C in 3.7% formaldehyde, pelleted by centrifugation, resuspended in 0.1 microgram/ml 4,6-diamidino-2- phenylindole (DAPI) for 6 min, pelleted and rinsed with water 6 times, and mounted in 75% glycerol in 25% phosphate buffer (pH 8). Nuclei were counted at 800X using filters recommended for DAPI by the microscope manufacturer (Zeiss). Spores from zymodeme I (collected from several pine hosts throughout California) were predominantly (>75%) binucleate, while spores from zymodeme II (collected from lodgepole pine in the central Sierra Nevada) were predominantly uninucleate. Implications for the nuclear behavior of this endemic forest pathogen are discussed.


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