A Virtual Tour of the Bruns Lab Continues...

Sample Results


3100 data collection software view 3100 entire run gel view rflp gel

microsatellite gel
Microsatellite markers, also called short tandem repeat (STR) markers, are polymorphic DNA loci that contain a repeated nucleotide sequence. The repeat unit can be from 2 to 7 nucleotides in length. The number of nucleotides per repeat unit is the same for a majority of repeats within a microsatellite locus
AFLP
DNA fragments labeled with P33
AFLP
DNA fragements fluorescently labeled
What is Microsatellite Analysis?
Microsatellite loci are PCR amplified and the PCR products are then analyzed by electrophoresis to separate the alleles according to size. PCR-amplified microsatellite alleles can be detected using various methods, such as fluorescent dye labeling, silver staining, or fluorescent dye staining.
Amplified Restriction Fragment Polymorphism (AFLP) technology is a technique for fingerprinting genomic DNA. DNA fingerprinting is used to visualize DNA polymorphisms between samples. These fingerprints may be used as a tool for determining the identity of a specific DNA sample or to assess the relatedness between samples. Fingerprints are also used as the source for genetic markers to generate linkage maps or to identify molecular markers linked to phenotypic traits and/or genetic loci.

TECHNIQUES FROM A BYGONE ERA
dot blot isozyme gel Before the acquisition of the 3100, sequencing data was obtained using an ABI PRISM 377.

Single Strand Conformational Polymorphism SSCP is a screening technique that allows one to detect sequence based polymorphisms. For an overview of the principle behind SSCP, click here.
35S sequencing gel SSCP gel

The tour continues: take a stroll through Point Reyes and visit one of our study sites before the devistating Mount Vision fire.
the beginning sample results point reyes fire introduction
limantour road disturbance plots bp plots species diversity plots
construction post fire - sd plots post fire - bp plots sierra nevada 1995
sierra july 1998 sierra august 1999 sierra fowm 2000 santa rosa island 2001
blodgett 2002 tomales point 2004 blodgett 2004 mclaughlin 2006
mushrooms mycorrhizae part I mycorrhizae part II rust fungi
post fire fungi Bruns Lab home

Last updated: Monday May 24 2004