UC Berkeley Forest Pathology and Mycology 
Laboratory City Forest
December 22, 2014
 
Testing for Sudden Oak Death - PCR Method

DNA Extraction
The polymerase chain reaction (PCR) method is used to detect the presence of P. ramorum in plant material. Scientific paper. Infected plant material is placed in a sample tube with a small glass bead, freeze dried, and pulverized in a tissue homogenizer.
The plant material is extensively processed to extract, isolate, and purify the target DNA. The DNA containing solutions are transfered to a microtiter plate to increase efficiency and assay throughput.
PCR Setup
Real Time PCR Machine
The thermocycler (Real-Time PCR machine) is used with P. ramorum-specific primers to amplify and simultaneously quantify regions of the DNA that are unique to P. ramorum. Scientific articles: Hayden 2006, Chandelier 2006, Martin 2009.

Pros and Cons of the PCR method:
Pro:
- The gold standard for P. ramorum detection.
- Highly specific for species identification.
- Eliminates false negative and false positive results.

Con:
- Expensive compared to culturing and EIA methods.
- Requires specialized equipment and training.
- Relatively slow compared to EIA methods.

Jump to Other SOD Diagnostic Methods:
Immunostrips - Culturing - EIA Analysis - PCR Analysis

The UC Berkeley Forest Pathology and Mycology Laboratory does not provide a SOD testing service. For information on SOD testing please visit www.suddenoakdeath.org


This activity possible thanks to funding from:
USDA Forest Service, State and Private Forestry
The Gordon and Betty Moore Foundation
Agdia, Inc. http://www.agdia.com

Disclaimer: Mention of any company, trade name, or commercial product does not constitute endorsement by the University of California or recommendation for use.



Home  |   Research  |   Publications  |   Diagnosis  |   Treatment
Links  |   Contact  |   UCB Courses  |   Featured



Please report any problems to the
Lexis Nexis Search