Amplification and sequencing of DNA from fungal herbarium
Bruns, T D; Fogel, R; Taylor, J W
Department of Plant Pathology, University of California
Mycologia. 1990 82(2):175-184.
We have utilized the polymerase chain reaction to amplify DNA from
35 collections of dried fungi obtained from four different herbaria.
Sixteen of these samples were subsequently sequenced. The collections
had been dried and stored under a variety of conditions for
1-50 years. DNA was extracted from 5-30 mg of ground tissue and a
specific fragment of the mitochondrial large subunit ribosomal RNA
gene was amplified. Sequences obtained from DNA cloned from a culture
and those from DNA amplified from the corresponding dried voucher
collection were found to be identical. Controls lacking DNA failed
to produce amplified products. This application of the polymerase
chain reaction increases the value of fungal herbarium specimens by
enabling their use in molecular systematic and population genetic
studies, and it creates new curatorial demands.