Differentiation of intersterility groups and geographic
provenances among isolates of Heterobasidion annosum
detected by random amplified polymorphic DNA assays.
Garbelotto, M; Bruns, T D; Cobb, F W; Otrosina, W J
Department of Environmental Science, Policy
University of California, Berkeley, CA 94720
Canadian Journal Of Botany. 1993 71(4):565-569.
Random amplified polymorphic DNAs were correlated with the
intersterility group and the geographic provenance of 36 isolates of
Heterobasidion annosum from North America and Europe and of one
herbarium collection of basidiocarps from California. This technique
is very precise and yields higher resolution than previous studies
implementing techniques such as isozyme electrophoresis and
restriction fragment length polymorphisms. Random amplified
polymorphic DNAs revealed differentiation among the following
geographic groups and intersterility groups: western North American P,
eastern North American P, European P, North American S, Scandinavian
S, Italian S, and Italian F. This is the first report on
differentiation between eastern and western North American P
isolates as well as between northern and southern European S
isolates. Successful amplification of one dry basidiocarp suggests
that random amplified polymorphic DNAs may be used to improve
epidemiological and population studies of this pathogen.
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