Molecular Ecology Notes
Volume 5 Issue 3 Pages 608  - 610.

September 2005

Isolation and characterization of microsatellite loci from the truffle-like ectomycorrhizal fungi Rhizopogon occidentalis and Rhizopogon vulgaris

Lisa C. Grubisha1, Annette Kretzer2 and Thomas D. Bruns3
1 Institute of Arctic Biology, 311 Irving 1 Building, University of Alaska, Fairbanks AK 99775-7000
2 SUNY College of Environmental Science and Forestry, Faculty of Environmental and Forest Biology, One Forestry Drive, Syracuse NY 13210-2788
3 Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley CA 94720-3102

We have isolated and characterized five microsatellite loci from Rhizopogon occidentalis and six loci from Rhizopogon vulgaris (Boletales, Basidiomycota). Microsatellite variation was assessed using 32 R. occidentalis and 48 R. vulgaris individuals from four populations in California. The number of alleles across populations ranged from two to 10 for R. occidentalis and three to eight for R. vulgaris. Expected heterozygosity values within populations ranged from 0.00 to 0.85 for R. occidentalis and 0.00 to 0.75 for R. vulgaris. These are the first microsatellite loci isolated for R. occidentalis and R. vulgaris and will be useful in the examination of their population genetic structure.

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