“Protein Purification Using Immobialized Metal Affinity Chromatography (IMAC) and Size Exclusion Chromatography”

Affinity chromatography is often used as a purification technique to separate a target molecule from a crude feedstock. This method of action is a reversible interaction between a protein and a specific ligand attached to the chromatography matrix. This ligand binds to the protein of interest while unbound materials are washed through the column. The purified sample is collected and purified further (if needed) then tested for stability within its given buffer. My project focused on the purification of a specific protein (Protein “One”), and required the use of two specific types of chromatography: immobilized metal affinity chromatography and size-exclusion chromatography. 

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Claudia is a Neuroscience major at UCLA. She attended Skyline College. She plans to enter an MD/PhD program at a medical school. Besides serving as secretary for the SHPE (Society of Hispanic Professional Engineers) Chapter, she is also a member of the Honors Transfer Program and MESA at Skyline. Claudia has presented her research at SFSU, Stanford University, Genentech and at the SACNAS National Conference. Her research has received first place awards at the BA STAR Conference at CSU Sacramento and the American Society of Microbiology Conference.