Coast redwood (Sequoia sempervirens) is an iconic tree species in California, and the tallest conifer in the world. However, because of the large and complex genome of this species (26.5 GB), genetic and genomic studies of this species have been limited. This project aims to develop new single nucleotide polymorphism (SNP) arrays for the species using foliage material collected in summer 2021 for use in landscape and population genomics analysis.
Previous studies of genetic diversity in coast redwood were conducted using either allozyme or microsatellite markers, thus making this the first study to explore population structure in coast redwood using higher resolution next-generation sequencing.
The student will work with Alexandra Sasha Nikolaeva (Ph.D. Candidate) to extract high molecular weight DNA from redwood foliage samples. Subsequently, the studnet will help prepare libraries for Illumina sequencing.
The student should preferably have experince working in a wet lab