Submitted by Sabeeha Merchant on
The primary objective of this project is to utilize molecular biology approaches in identifying regulatory details of the nutritional Cu signaling pathway in Chlamydomonas. CRR1 is a transcription factor that directly regulates more than 63 genes in Cu-deficiency through sequence-specific binding to Cu response elements (CuRE) associated with the target genes. In the presence of Cu, CRR1 is rapidly degraded. Resolving the mechanism of degradation and how Cu signals to CRR1 is the focus of this project.
The student will be involved in hands-on laboratory work, including molecular biology and biochemical experiments and analyses (SDS-PAGE immunoblot, photosynthetic parameters) and handling of algal strains (preparation of cell culture media, maintenance, phenotypic characterization of mutant strains). The student may participate in the data analysis of large-scale multi-omics experiments. The student will participate in regular group meetings and discuss experimental results with the mentor.
We are looking for a highly motivated student with a strong passion for scientific research. Attention to detail, accurate protocol adherence, and strict compliance to safety guidelines are expected from the student. The student should also have effective communication skills and the ability to collaborate within a team environment.