Submitted by Patrick M Shih on
Robust processes for capturing atmospheric carbon will be at the heart of efforts to create bio-based alternatives to fossil-derived products like fuel and plastic. Recent efforts to optimize biological carbon capture for bioproducts have mostly focused on plants, which use the Calvin-Benson-Bassham (CBB) cycle to “fix” carbon dioxide. However, the CBB cycle is noted for its low efficiency, which has sparked interest in multiple other carbon fixation pathways that exist in diverse microbes, some of which may offer improved carbon-capturing performance for the bioeconomy.
The primary project will examine the 3-hydroxypropionate (3HP) bicycle, one such alternative microbial carbon fixing metabolism. The goal of the project is to use genetic engineering to recreate the 3HP bicycle within a model cyanobacterium, Synechocystis sp. PCC6803. Although Synechocystis normally fixes carbon using the CBB cycle, it has a uniquely flexible metabolism that may allow it to use the 3HP bicycle, if supplied with the right genes. Recreating the 3HP bicycle in vivo will shed light on how this metabolism evolved and will also serve as a proof of concept for transferring carbon fixation pathways between organisms, laying the foundation for the implementation of efficient carbon fixation pathways in bioproduction hosts such as algae or even higher plants.
The primary project will involve working with a graduate student to clone the genes of the 3HP bicycle into Synechocystis. Mentees will learn and apply 1) PCR for cloning and genotyping 2) DNA assembly techniques such as Gibson assembly 3) Generation of transgenic Synechocystis lines using assembled DNA and 4) Analysis of transgenic Synechocystis using techniques such as growth phenotyping, spectrophotometric assays, and HPLC. Additional biochemical techniques, as well as opportunities for independent research projects, may also be incorporated based on mentee interest and as the project develops.
Minimum qualifications: Biology 1A or 1B (may be taken concurrently), 3.0 GPA, and interest in genetics. Hours are negotiable, but a commitment of at least 8 hours per week is expected. Enthusiasm for research is necessary, and care-to-detail and commitment to scheduled work times are critical.