Submitted by Brian Staskawicz on
We are interested in elucidating the immune system of plants. We have been focusing on the plant protein NRG1, which plays essential roles in plant defense against pathogens. Although NRG1 was identified about ten years ago, its molecular functions in plant immunity remains largely unclear. In order to elucidate the molecular mechanisms of NRG1, we have been identifying NRG1-interaction proteins by yeast two hybrid (Y2H) screening and co-Immunoprecipitation in concert with mass spectrometry. We have identified several NRG1-interaction proteins. We will employ Virus Induced Gene Silencing (VIGS) technology to silence the candidate genes in tobacco and test the immune responses of these silenced lines.
In addition, we generated Arabidopsis DEX-inducible NRG1 transgenic plants and found that DEX-induced NRG1 expression caused autoimmunity in Arabidopsis. In order to find novel components that function in NRG1-mediated plant immunity pathway, we carried out a forward genetic screening in M2 populations of EMS-mutated NRG1 transgenic seeds. Some mutant lines exhibited compromised autoimmunity. We will continue to screen the mutant library and map candidate genes by crossing and whole genome sequencing.
The undergraduate student will help to construct VIGS vectors, plate Arabidopsis seeds and take care of tobacco plants. In addition, the individual will have the opportunity to learn basic techniques for biological research, such as PCR, gel electrophoresis, plasmid extraction, cloning, yeast transformation, pathogen infection, phenotype observation and data collection.
Student must be a 2nd year or older with a major in the biological sciences, especially in microbiology or plant biology majors. Previous research experiences are not required, but basic laboratory training is essential, such as calculations of molarity and serial dilutions. Expected to work at approximately 8 hours a week (schedule can be flexible, but class schedules that allow 2-3 hour blocks of time are best).