The overall goals of the proposed research are: i) To move landscape genomics from a correlative approach to one in which gene function can be tested using CRISPR/Cas9 gene editing and, ii) To identify sequence variation in two putative adaptive genes among populations of an ecologically and economically important western North American tree species (sugar pine), distributed across an environmental gradient and, to test the role of variation in these genes in drought and temperature tolerance.
To extract DNA from needles of populations of Pinus lambertiana using a Genomic DNA Isolation Kit or CTAB method. Genotyping using the primers designed by other lab members in order to identify possible allelic variation among populations. To conduct PCRs and gel electrophoresis.
Experience with DNA extraction, PCRs and gel electrophoresis